Different types of culture media used in microbiology pdf. The concept of selective media is usually a simple one. Get a printable copy pdf file of the complete article 676k, or click on a. Selective mediaedit selective media are used for the growth of only selected microorganisms. This study discusses the optimisation of a selective and differential. Selective and differential media for isolation and. Enriched, selective, and differential media youtube. In a microbiology lab you would use bergeys manual of determinative bacteriology. Media used for bacterial growth microbiology lumen learning. Understand the use of antibiotics on microorganisms. A new selective differential medium for the isolation ofstenotrophomonas formerlyxanthomonas maltophilia was developed. Lab biochemical tests and selectivedifferential media. The ability of the five selective media to recover 1,500 enterotoxigenic staphylococci three strains per ml from three sterile foods in the presence of 10 strains of contaminating bacteria added at the 0, 10 5, and 10 6 levels per milliliter was also studied and analyzed statistically.
Sometimes selective and differential functions are combined in one medium. For example, organisms that can utilize a given sugar are easily screened by. Development of an improved selective and differential. They also incorporate chemical compounds that, following inoculation and incubation, will produce a characteristic change in the appearance in bacterial growth, as well as the medium. A new differential and selective medium for the isolation of members of the proteeae.
Types of media selective and differential media flashcards. Selective media use specific growth characteristics of a microorganism to selectively grow. Most of this socalled selective growth media contain a protein source, often a hydrolysate of casein, and a fermentable sugar, like lactose or glucose. Let us say that the specimen of interest is one cell in a million. Key difference selective vs differential media microorganisms are often grown under laboratory conditions for various purposes. What type of media is kf streptococcal and what is the mode of action i. Pmc free article dowell vr, jr, hill eo, altemeier wa.
Often, in a clinical setting, the most helpful type of medium is one that is both selective and differential. Evaluation of differential and selective media for isolation of aeromonas and plesiomonas spp. On the basis of reducing background noise, an optimum basal medium and nitrogen source were selected. When dealing with unknown microorganisms and their identification the use of special purpose media is a key factor in success. Differential media differential media allows us to distinguish between different microbes that grow on the same medium.
Pdf schizosaccharomyces selective differential media. For example, if a microorganism is resistant to a certain antibiotic, such as ampicillin or tetracycline, then that antibiotic can be added to the medium in order to prevent other cells, which do not possess the resistance, from growing. Different types of selective and differential media, and identification of microorganisms depending on growth or colour change. Other components of the medium such as antibiotics, salt. Select print, and, when the print screen comes up, go to the print handling options. The best way for students to print out the powerpoint show is to download the pdf version. Oxidationfermentation test using of basal media with glucose.
Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms. Gain more knowledge about selective media and differential media. Comparative evaluation of five selective and differential. Thus, the phrase recombinant dna refers to an organism created in the lab by adding dna from another species. It is not selective, and will grow many different types of microbes. Differential media typically display some type color change in the presence of certain bacteria. Selective media are used for the growth of only selected microorganisms. The use of selective and differential plating media is a simple method for the isolation of salmonella spp. Difference between selective and differential media. Eosin methylene blue contains dyes that are toxic for grampositive bacteria. Development of a novel selective and differential medium. It is used because it is able to attach the free nucleotides to. Practice use of the catalase test, coagulase and the oxidase test.
Selective and differential media were designed for each species of pityrosporum. The 3 different types of media that will be used in this experiment are selective media. Selective differential media growth medium prokaryote. Occasionally an indicator, like neutral red or bromo cresol purple, is added to detect the acid produced by the fermentation process.
Colonies of the lactose fermenters are red or pink, while those of nlfs are colourless. This is the difference between selective media and differential media. Selective and differential media use of selective and. Download pdf version of selective vs differential media. Biochemical tests and selective and differential media objectives utilize both selective and differential media in growth and identification of bacteria. Compared withxanthomonas maltophilia selective medium xmsm, via agar was. It is the selective and differential medium for coliforms.
In this course you will encounter many types of growth media. Some of the ingredients in the medium favor the growth of the targeted bacteria. Selective media is often used when one is trying to isolate a specimen of interest from a mixed culture. Reading comprehension ensure that you draw the most important information from the related lesson on differential and selective media in microbiology distinguishing differences compare the. Pdf evaluation of differential and selective media for. Selective media contains certain compounds or chemicals that favor the growth of certain species of bacteria. The medium, via agar, contained vancomycin, imipenem, and amphotericin b as selective agents and incorporated a mannitolbromothymol blue indicator system. Comparison of selective and differential media for the. Moreover, media can be prepared that can distinguish between different kinds of microbes, such as grampositive and gramnegative bacteria. Use of selective and differential media to grow and. Bio 220 microbial growth media napa valley college. Tryptic soy agarbroth and nanb nutrient agarbroth for example.
Use of selective and differential media many different types of media are used in the microbiology laboratory. Selective growth media for differentiation and detection. Selective media selective media is designed to suppress the growth of unwanted bacteria and encourage the growth of the desired microbes. Ym yeast extract, malt extract agar has a low ph, deterring bacterial growth macconkey agar is for gramnegative bacteria hektoen enteric agar is selective for gramnegative bacteria. Various selective and differential media were evaluated for their suitability to provide reliable counts of lactobacillus acidophilus, bifidobacterium spp. Differential media distinguish the microorganisms by allowing them to produce visible growth pattern or different characteristics on the media. A wide variety of selective and differential media have been developed for this purpose, including palcam, oxford agar, and modified oxford agar mox 10 12. For example, macconkeys agar differentiates enterobacteria that ferment lactose from nonlactose fermenters nlfs. Selective and differential media, unknown id enzyme based tests oct. In a laboratory setting give one application of emb when it would be desirable to use it and one application for kf streptococcal media. A new selective differential medium for isolation of. Selective and reversible inhibition of the synthesis of bacterial deoxyribonucleic acid by phenethyl alcohol. These types are typically considered nonselective due to the fact. Selective and differential media are used to isolate or identify particular organisms.
Are you confused by the concepts of selective and differential media. Functionally, selective media permit certain types of organisms to grow while inhibiting the. Selective and differential medium for the primary isolation of. Blood agar bap is another type of differential medium. The second type is differentialselective media, which is the media that can distinguish among morphologically and biochemically related groups of organisms. Identify the different types of media used in this lab perform a variety of biochemical tests and analyze their reactions and their results introduction artificial media represents an environment created. In general, these media can be divided into several categories, including nutritive, differential, and selective. Ill try to explain in simple terms and i will give clear examples.
There are many types of media used in the studies of microbes. Selective media reported to develop only single type of colonies, demonstrated two types of colonies with some yoghurts, making it difficult to conclusively enumerate the. Selective differential media selective media population ingredients selected determines what grows msap. Selective and differential media several examples of commonly used bacteriological media, as well as examples with one or more types of bacteria cultured on them are shown in the module. This term refers to something formed by combining existing elements in a new combination. Understand the selective and differential aspects of derstand.
The main difference between selective and differential media is that selective media are used to isolate a particular strain of microorganisms whereas differential media are used to identify and differentiate a closelyrelated group of microorganisms. Selective differential media free download as powerpoint presentation. The different types of media used to isolate, identify and grow the bacteria of interest, are selective media, differential media and enriched media. Differential media, unlike selective media, does not kill organisms. Each can be enlarged by clicking once on the image. This bacterium showed strong fluorogenic characteristics clearly distinguishable from other microorganisms. Two types of media with similar implying names but very different functions, referred to as selective and differential media, are defined as follows.
Learn vocabulary, terms, and more with flashcards, games, and other study tools. Identify and describe culture media for the growth of bacteria, including examples of. Selective and differential media can be combined and play an important role in the. Selective and differential media is extremely useful when examining mixed bacterial cultures since it provides. Solid media generally contain agar at a concentration of 1. Certain media work as both selective and differential media. Emb agar is selective media used for the isolation and differentiation of enteric bacilli, especially coliforms, in clinical specimens, water, and dairy products. Nutritive media are defined as media types that support the growth of a wide range of microorganisms. Selective and differential media flashcards quizlet. Growing microbes is feasible only if they are provided with a suitable culture. Differential medium definition of differential medium by. A wide variety of selective and differential media has been developed for this purpose, including xylose lysine desoxycholate agar xld, hektoen enteric. Use of differential, selective, and enriched media. Selective and differential media biology libretexts.
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